Medical Sciences Portal and Biliary Hypertension as the Cells Proliferation Trigger (Landmarks for Future Investigations)

ABSTRACT. The outcomes of the study demonstrated that the violent activation of cellular proliferation in liver tissue can be triggered by partial hepatectomy (PH), as well as by common bile duct ligation (BDL). However, the causes of the mentioned activation are not still clearly defined. 54 white Wistar male rats of II postpubertal age with weight 200-220 g were included in PH or BDL models. In PH setting, pressure in portal vein was measured before and immediately after PH, as well as at 6th, 24th, 48th, and 144th hours past the operation. Mitotic bodies were counted immediatly after PH, and at 6th, 32nd, 72nd and 144th hours after the operation. In BDL setting, the pressure in common bile duct was measured before and at 1st, 3rd, 72nd, 96th and 120th and 144th hours after its ligation, and pressure in portal vein in this same setting was measured at 1st, 6th, 24th, 72nd, 144th hours past the operation. Mitotic bodies were counted at 24th, 48th, 72nd, 96th and 144th hours after BDL. On the basis of data obtained and analyzed the following conclusions are made: portal and biliary hypertension appeared to serve as a trigger for cellular proliferative activity in PH and BDL settings. The comprehensive evaluation of the processes involving liver regeneration following partial hepatectomy and post-BDL biliary ductular proliferation, hence requires not only the reduction of intervals between experimental data and refinement of the morphological and/or molecular/biological techniques of the study, but also the development of new experimental models with novel approaches. © 2009 Bull. Georg. Natl. Acad. Sci. Key words: portal hypertension, biliary hypertension, ductular reaction, hepatocyte, mitosi