Screening of Micrococcus Sp from Oil Contaminated Soil with Reference to Bioremediation

Abstract: For the present study the soil samples were collected from three different oil contaminated sites at Mayiladuthurai and Nagappattinam District in India. Soil samples were used to analyze total nitrogen, phosphorus, potassium, zinc, copper, iron, manganese and electrical conductivity Ec (dSM). The temperature1 was found to vary in the three different soil samples. The macronutrients (nitrogen, phosphorus, potassium) and micronutrients (zinc, copper, iron and manganese) were also different in three soil samples. Seven bacteria were isolated from the oil contaminated soil samples. From the above isolates Micrococcus sp was taken for the present study. Biosurfactant activity of Micrococcus sp was tested in petrol and diesel, which showed Moderate activity. BATH assay was determined for the bacterial cells. The growth potential of hydrocarbon utilizing bacteria on petrol and diesel was also observed. Biodegration activity was tested by liquid culture characteristics and emulsification activity in two hydrocarbon substrates by using Micrococcus sp. These beneficial microorganism (Micrococcus sp) used in my study is to cleanup the oil contaminated sites and prevent the soil from unwanted substances. Key words: Soil samples Hydrocarbon Degradation INTRODUCTION components. Many microorganisms have the ability to Micrococcus is a genus of bacteria in the energy. Such microorganisms are widely distributed i