Chromosomal and Somatic Karyotype Analysis in some genotypes of Rosa damascena mill (Rosaceae)

damascena mill (Rosaceae) Rose is a shrub belonging to the family Rosaceae (Rose family) and the genus Rosa, species damascena variety common names damask.. This is the first report on chromosome number and karyotype for 15 Rosa damascena mill genotypes. Fifteen Rosa damascena mill populations were sampled for this study. To observe metaphase plates, root-tip meristems were immersed in 0.01 M α-Bromo naphthalene at 4°C for 2-3 h and then fixed in ethanol: acetic acid (3:1, v/v) at 60°C for 8 min, and finally stored in %70 ethanol at 4°C. To observe metaphase chromosomal, meristematic tissue was stained with %0.02 (w/v) Aceto-orcein. For staining, root-tip were put on Aceto-urcein at room temperature for 35-40 min. The roots were then gently squashed in a drop of %45 acetic acid. For the cytological investigation, images were captured with a BX50 Olympus camera. For numerical karyotype analysis, chromosomes from five metaphase were measured for Rosa damascena mill were identified and ordered according to their long length, short length, total length (TL), Form percentage (F% = short length / tota