Habitat Fingerprints for Lake Superior Coastal Wetlands Derived from Elemental Analysis of Yellow Perch Otoliths

Abstract.—Assessing the ecological importance of coastal habitats to Great Lakes ecosystems requires an understanding of the ecological linkages between coastal and offshore waters. Elemental analysis of fish otoliths has emerged as a powerful technique that can provide a natural tag for determining nursery area affiliation, population structure, and movement of individual fish. Since the elemental composition of fish otoliths reflects some of the environmental conditions under which a fish was reared, otolith chemistry can record differences in ambient water conditions specific to habitats used during a fish’s life history. Although few studies have been conducted in freshwaters, trace element analysis of marine fish otoliths has proven useful in identifying the chemical signatures unique to particular spawning and nursery habitats. To examine the utility of this method in freshwater, sagittae were removed from 275 young-of-the-year yellow perch Perca flavescens captured from eight wetlands in western Lake Superior during August 2001. They were analyzed for Ca and 13 minor and trace elements using inductively coupled plasma mass spec-trometry (ICPMS) and inductively coupled atomic emission mass spectrometry (ICPAES). Otolith concentrations of Ba, K, Mg, Mn, Na, and Sr differed significantly among wetlands (ANOVA, P, 0.001). Interwetland differences were also pronounced when analyzed as a multivariate fin-gerprint (MANOVA, P, 0.001). Discriminant function analysis revealed relatively distinct chem