Isolation and Analysis of a Novel Gene, HXC-26, Adjacent to the rab GDP Dissociation Inhibitor Gene Located at Human

We screened potential promoter regions from./Votl-linking cosmid clones mapped on human chromo-some Xq28 region with our constructed trapping vector and isolated six fragments containing transcription activity. Using one of the obtained fragments as a probe, a novel gene was isolated by screening a human skeletal muscle cDNA library. The isolated cDNA, termed HXC-26, contained an open reading frame of 975 nucleotides encoding 325 amino acids (38,848 Da). The HXC-26 gene was composed of 13 exons that span ~ 8 kb. Several potential GC boxes were found in the putative promoter region, but no typical TATA box. The HXC-26 gene associated with a CpG island was located adjacent to the rab GDP dissociation inhibitor (GDI) gene. Key words: cDNA cloning; human chromosome Xq28; HXC-26 To advance the large-scale sequencing, it is essential to construct a more precise physical map of the hu-man genome. Through this process, we can find func-tional units in the genomic DNA. Moreover, we have clues to understand the principles of genome organizatio