Collaborators:

  • David Lampe
  • Carol Lauzon
  • Thomas A. Miller
Publication date
July 2015

Abstract

Strains of Alcaligenes xylosoxidans denitrificans (Axd) that secrete anti-Xylella factors are being developed for use in a strategy to prevent the spread of Xf or possibly to cure infected grapevines. We built constructs that fused the last 60 amino acids of the autotransporter α-hemolysin from E. coli to test proteins. These were two different forms of anti-BSA single chain antibodies (scFvs) which are surrogates for anti-Xylella effector proteins. These proteins were efficiently secreted from E. coli when co-expressed with the proteins HlyB and HlyD. HlyB, HlyD, and TolC together form the membrane structure used by α-hemolysin to cross both the inner and outer membrane of the cell. We report here on efforts to move this system into Axd, a...

Extracted data

We use cookies to provide a better user experience.