Strains of Alcaligenes xylosoxidans denitrificans (Axd) that secrete anti-Xylella factors are being developed for use in a strategy to prevent the spread of Xf or possibly to cure infected grapevines. We built constructs that fused the last 60 amino acids of the autotransporter α-hemolysin from E. coli to test proteins. These were two different forms of anti-BSA single chain antibodies (scFvs) which are surrogates for anti-Xylella effector proteins. These proteins were efficiently secreted from E. coli when co-expressed with the proteins HlyB and HlyD. HlyB, HlyD, and TolC together form the membrane structure used by α-hemolysin to cross both the inner and outer membrane of the cell. We report here on efforts to move this system into Axd, a...
Pierce’s disease (PD) is an incurable disease of grapevine caused by strains of Xylella fastidiosa. ...
Aims: Identification of a gene for self-protection from the antibiotic-producing plant pathogen Xant...
Xylella fastidiosa is a xylem-limited, Gram-negative, gammaproteobacterium, and the causal agent of ...
Reporting Period: The results reported are from work conducted October 2004 to October 2005. Several...
It is likely that the development of grapevine cultivars resistant to Xylella fastidiosa Xf presents...
Symbiotic control employs symbiotic bacteria to deliver anti-pathogen compounds to disrupt transmiss...
The aim of this project is to construct and express in grapevine, a protein or protein chimera (“ant...
Xylella fastidiosa (Xf) is a gram-negative xylem-limited bacterium and causative agent of Pierce’s d...
The principal objective of this project is to construct and express in test plants, and then in grap...
A bacterium isolated from Homalodisca coagulata Say, found also to inhabit xylem of citrus and grape...
Xylella fastidiosa is an emerging problem among infective agents threatening crops of commercial int...
We have developed an autonomously replicating Xylella fastidiosa (Xf)/E. coli plasmid that efficient...
In previous work, marker exchange mutagenesis of the single tolC gene in Pierce’s Disease (PD) strai...
Xylella fastidiosa (Xf) is a xylem-limited bacterium, regulated as a quarantine pest, that is causin...
Xylella fastidiosa is a xylem-limited, insect transmitted bacterium that causes fatal diseases in ec...
Pierce’s disease (PD) is an incurable disease of grapevine caused by strains of Xylella fastidiosa. ...
Aims: Identification of a gene for self-protection from the antibiotic-producing plant pathogen Xant...
Xylella fastidiosa is a xylem-limited, Gram-negative, gammaproteobacterium, and the causal agent of ...
Reporting Period: The results reported are from work conducted October 2004 to October 2005. Several...
It is likely that the development of grapevine cultivars resistant to Xylella fastidiosa Xf presents...
Symbiotic control employs symbiotic bacteria to deliver anti-pathogen compounds to disrupt transmiss...
The aim of this project is to construct and express in grapevine, a protein or protein chimera (“ant...
Xylella fastidiosa (Xf) is a gram-negative xylem-limited bacterium and causative agent of Pierce’s d...
The principal objective of this project is to construct and express in test plants, and then in grap...
A bacterium isolated from Homalodisca coagulata Say, found also to inhabit xylem of citrus and grape...
Xylella fastidiosa is an emerging problem among infective agents threatening crops of commercial int...
We have developed an autonomously replicating Xylella fastidiosa (Xf)/E. coli plasmid that efficient...
In previous work, marker exchange mutagenesis of the single tolC gene in Pierce’s Disease (PD) strai...
Xylella fastidiosa (Xf) is a xylem-limited bacterium, regulated as a quarantine pest, that is causin...
Xylella fastidiosa is a xylem-limited, insect transmitted bacterium that causes fatal diseases in ec...
Pierce’s disease (PD) is an incurable disease of grapevine caused by strains of Xylella fastidiosa. ...
Aims: Identification of a gene for self-protection from the antibiotic-producing plant pathogen Xant...
Xylella fastidiosa is a xylem-limited, Gram-negative, gammaproteobacterium, and the causal agent of ...