PTC Effect of Physical Factors on Micropropagation of

Anthurium shoot tips were successfully micropropagated on either agar- or rockwool-based substrates in either conventional glass bottles with (Milliseal® added) or without aeration, or within a Neoflon ® PFA film Culture Pack®, with modified half strength of MS as the medium of choice. Rockwool proved to be a far better substrate than agar, while aeration, provided either by the presence of a Milliseal ® on the cap or by the use of Neoflon ® PFA film, enhanced the growth, rooting and subsequent acclimatization (100%) of this popular ornamental plant. A scaled-up model was then applied for the mass production (96 plants per vessel) of Anthurium with similar positive growth results