SNP Cutter: a comprehensive tool for SNP PCR-RFLP assay design". Nucleic Acids Res. 33 (Web Server issue): W489-92. doi:10.1093/nar/gki358. PMID 15980518. No:14 – New Trends in Intelligent Technologies Authors' Information Francisco J. Cisneros – Natural

The Polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) is a relatively sim-ple and inexpensive method for genotyping single nucleotide polymorphisms (SNPs). It requires minimal investment in instrumentation. Here, we describe a web application, ‘SNP Cutter, ’ which designs PCR–RFLP assays on a batch of SNPs from the human genome. NCBI dbSNP rs IDs or formatted SNPs are submitted into the SNP Cutter which then uses restriction enzymes from a pre-selected list to perform enzyme selection. The program is capable of designing primers for either natural PCR–RFLP or mismatch PCR–RFLP, depending on the SNP sequence data. SNP Cutter generates the information needed to evaluate and perform genotyping experi-ments, including a PCR primers list, sizes of original amplicons and different allelic fragment after enzyme digestion. Some output data is tab-delimited, there-fore suitable for database archiving. The SNP Cut-ter is available a