P-selectin–Coated Microtube for Enrichment of CD34 Hematopoietic Stem and Progenitor Cells from

topoietic stem and progenitor cells (HSPCs) is impor-tant in transplantation therapies for hematologic dis-orders and in basic stem cell research. Primitive CD34 HSPCs have demonstrated stronger rolling adhesion on selectins than mature CD34 mononuclear cells (MNCs).We have exploited this differential rolling be-havior to capture andpurifyHSPCs frombonemarrow by perfusingMNCs through selectin-coatedmicrotubes. METHODS: Bone marrowMNCs were perfused through the cell-capture microtubes coated with adhesionmol-ecules.Wewashed the device lumen and visualized and estimated captured cells by video microscopy. Adher-ent cells were eluted by high shear, calcium-free buffer, and air embolism. We used immunofluorescence staining followed by flow cytometry to analyze CD3