Nucleotide specificity of the enzymatic and motile activities of dynein, kinesin, and heavy meromyosin

Abstract. The substrate specificities of dynein, kine-sin, and myosin substrate turnover activity and cyto-skeletal filament-driven translocation were examined using 15 ATP analogues. The dyneins were more se-lective in their substrate utilization than bovine brain kinesin or muscle heavy meromyosin, and even differ-ent types of dyneins, such as 14S and 22S dynein from Tetrahymena cilia and the/~-heavy chain-contain-ing particle from the outer-ann dynein of sea urchin. flagella, could be distinguished by their substrate specificities. Although bovine brain kinesin and mus-cle heavy meromyosin both exhibited broad substrate specificities, kinesin-induced microtubule translocation varied over a 50-fold range in speed among the vari-ous substrates, whereas heavy meromyosin-induced ac