Today’s research demands fast identification of potential diagnostic and therapeutic targets. We describe a novel phage display strategy to identify disease-related pro-teins that are specifically expressed in a certain (dis-eased) tissue or cells. Phages displaying antibody frag-ments are selected on complex protein mixtures in a two-step manner combining subtractive selection in solution with further enrichment of specific phages on two-dimen-sional Western blots. Targets recognized by the resulting recombinant antibodies are immunoaffinity-purified and identified by mass spectrometry. We used antibody frag-ment libraries from autoimmune patients to discover apo-ptosis-specific and disease-related targets. One of the three identified targ...
The immune system is an important part of the physiology of higher organisms. In the immune system a...
Abstract M13 filamentous bacteriophage has been used in displaying disease-specific antibodies, biom...
With the advent of modern technologies enabling single cell analysis, it has become clear that small...
Phage display represents an invaluable tool to study autoimmune diseases. The side effects of immuno...
Phage display technology (PDT), a combinatorial screening approach, provides a molecular diversity t...
One way to improve the therapeutic index of anti-cancer agents would be to target them to the tumour...
Phage display bypasses the immune response by bringing the entire immune repertoire to a target anti...
This thesis has focused on the development of methods and their applications for the identification ...
Since its introduction almost 20 years ago, phage display technology has revolutionized approaches t...
Since the advent of phage display technology, dating back to 1985, antibody libraries displayed on f...
We report the construction and the use of a phage display human antibody library (>3 x 10(8) clones)...
Monoclonal antibodies (mAbs) have become one of the most important classes of biopharmaceutical prod...
7noDuring the last 20 years in vitro technologies opened powerful routes to combine the generation o...
Autoimmune diseases, which affect approximately 2-3% of the world population, are characterized by t...
The phage display is becoming a very powerful technology. Filamentous phages can present on their su...
The immune system is an important part of the physiology of higher organisms. In the immune system a...
Abstract M13 filamentous bacteriophage has been used in displaying disease-specific antibodies, biom...
With the advent of modern technologies enabling single cell analysis, it has become clear that small...
Phage display represents an invaluable tool to study autoimmune diseases. The side effects of immuno...
Phage display technology (PDT), a combinatorial screening approach, provides a molecular diversity t...
One way to improve the therapeutic index of anti-cancer agents would be to target them to the tumour...
Phage display bypasses the immune response by bringing the entire immune repertoire to a target anti...
This thesis has focused on the development of methods and their applications for the identification ...
Since its introduction almost 20 years ago, phage display technology has revolutionized approaches t...
Since the advent of phage display technology, dating back to 1985, antibody libraries displayed on f...
We report the construction and the use of a phage display human antibody library (>3 x 10(8) clones)...
Monoclonal antibodies (mAbs) have become one of the most important classes of biopharmaceutical prod...
7noDuring the last 20 years in vitro technologies opened powerful routes to combine the generation o...
Autoimmune diseases, which affect approximately 2-3% of the world population, are characterized by t...
The phage display is becoming a very powerful technology. Filamentous phages can present on their su...
The immune system is an important part of the physiology of higher organisms. In the immune system a...
Abstract M13 filamentous bacteriophage has been used in displaying disease-specific antibodies, biom...
With the advent of modern technologies enabling single cell analysis, it has become clear that small...
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