Mapping Functional Connectivity in Patients

Objective: The spatial distribution of functional connectivity between brain areas and the disturbance introduced by focal brain lesions are poorly understood. Based on the rationale that damaged brain tissue is disconnected from the physiological interac-tions among healthy areas, this study aimed to map the functionality of brain areas according to their connectivity with other areas. Methods: Magnetoencephalography recordings of spontaneous cortical activity during resting state were obtained from 15 consecutive patients with focal brain lesions and from 14 healthy control subjects. Neural activity in the brain was estimated using an adaptive spatial filtering technique. The mean imaginary coherence between brain voxels was then calculated as an index of functional connectivity. Results: Imaginary coherence was greatest in the alpha frequency range corresponding to the human cortical idling rhythm. In healthy subjects, functionally critical brain areas such as the somatosensory and language cortices had the highest alpha coher-ence. When compared with healthy control subjects, all lesion patients had diffuse or scattered brain areas with decreased alpha coherence. Patients with lesion-induced neurological deficits displayed decreased connectivity estimates in the corresponding brain area compared with intact contralateral regions. In tumor patients without preoperative neurological deficits, brain areas showing decreased coherence could be surgically resected without the occurrence of postoperative deficits. Interpretation: Resting state coherence measured with magnetoencephalography is capable of mapping the functional connec