FORMULATION & ANALYSIS OF LEVOFLOXACIN HEMIHYDRATE BY RP-HPLC

Objective(s) Developed and validate analytical method for quantitative determination of Levofloxacin tablet formulation. It was prepared by wet granulation method and evaluated by various physicochemical parameters. Analytical method was validating in different parameters like specificity, accuracy, linearity, precision, range, detection limit, robustness. Study in reversed phase high performance liquid chromatographic (RP-HPLC) method for the quantitative determination of Levofloxacin in bulk tablet and finished tablet. Materials and Methods: A shim-pack CLC-ODS column(25cm X 4.6mm, 5µm) and a mobile phase constituted of Buffer (850 ml of 0.05 (M) Citric acid monohydrate and 10 ml of 1 (M) ammonium acetate):Acetonitirile (150) were used. The flow rate was 1 ml/min and the analyses performed using ultraviolet (UV) detector at a wavelength of 293 nm using levofloxacin Pure drug as an internal standard. Separation was completed within 15 to 17 min. Results: The developed method showed good resolution between formulated tablet and standard drug. The standard solution of Levofloxacin was prepared to obtain the concentration of about 100 µg /mL