2006. Effect of diverse hormonal regimes on in vitro growth of grape germplasm

Two accessions of grape germplasm were tested on Murashige-Skoog media containing varying levels of BAP either or not containing IAA and NAA for in vitro growth parameters. It was observed that shoot number and shoot mass increased when the concentration of BAP was increased in a linear fashion. The shoot length of both the accessions showed a downward trend when the concentration of BAP was increased. The presence of IAA or NAA in the media, however, had a positive effect on the shoot length even in the presence of BAP (0.2 mg/l). It was possible to induce root induction on hormone free media for both the accessions used but the presence of NAA or IAA in the media had a profound effect on root induction of both the accessions. NAA was found to be more effective in root induction than IAA. Increased levels of BAP were found to inhibit root mass accumulation in both the accessions but the degree of inhibition was found to be accession specific