Comparative Studies on Diagnosis of Trypanosoma evansi in Experimentally Infected Goats

Abstract: The present study was conducted to evaluate the use of polymerase chain reaction (PCR) for detection of Trypanosoma evansi in experimentally infected goats. Special emphasis was paid to comparison with the other methodologies such as: parasitological techniques wet blood film (WBF), buffy coat technique (BCT), mouse inoculation (MI) and the diagnostic serological test (Card agglutination trypanosoma test (CATT). The diagnostic sensitivity of each test was also estimated. To achieve these purposes, ten adult female dry goats, 18-24 months old were used. The animals were verified to be free from T. evansi infection by mouce inoculation test. After the infection of the animals, blood samples were taken and used for performing the different analyses. This study revealed that application of PCR using T. evansi specific primers was the highes