P8 THE DERIVATION OF MOUSE EMBRYONIC STEM CELL LINES FROM A HYBRID STRAIN

The aim of this study was to establish germ-line competent ES cell lines from the hybrid mouse strain, B6D2F1. Methods The classical method1,2 to establish a mouse ES cell line was used with a slight modification. Without either the removal of the zona pellucida or immunosurgery, the 3.5 dpc blastocyst-stage embryos were placed directly into 24-multi-well dishes, precoated with mitomycin-C inactivated primary mouse embryonic fibroblasts (pMEF). The blastocyst embryos were cultured in Dulbecco modified Eagle medium (DMEM) high glucose, containing 2000 IU/ml leukemia inhibitor