Legume seed inoculation technology-a review

Inoculation of legume seed is an efficient and convenient way of introducing effective rhizobia to soil and subsequently the rhizosphere of legumes. However, its full potential is yet to be realised. Following widespread crop failures, the manufacture of high quality inoculants revolutionised legume technology in Australia in the 1960s. Many improvements to inoculants and the advent of an inoculant control service ensured that quality was optimised and maintained. Minimum standards for the number of rhizobia per seed were set after consideration of several factors including seed size and loss of viability during inoculation. Despite manufacturers ’ recommendations for storage and application of inoculants, there is a distinct lack of control over the inoculation process; hence the full potential of high quality products may not always be achieved. The efficacy of inoculation varies depending on several factors, all of which affect the number of viable rhizobia available for infection of legume roots. Increased numbers of viable rhizobia per seed by application of inoculant above the commercially recommended rate, results in a continued linear increase in nodulation and yield. Several studies have reported yield increases of up to 25%. However, applying higher quantities of inoculant is uneconomical and technically difficult. Alternatively, higher numbers of viable rhizobia per seed may be achieved by improving survival during seed inoculation. Despite recognition of the factors affecting survival of rhizobia on seed and a substantial demand for commercially pre-inoculated legume seed, poor survival is still a major concern. Desiccation, temperature and seed coat toxicity all influence survival of rhizobia on seed. Their adverse effects may be ameliorated by selecting tolerant rhizobial strains and legume seed cultivars with low toxicity or artificially, by the use of additives in the seed coating. The accumulation of th