Production of monoclonal and monospecific antibodies against non-capsid proteins of poliovirus

Non-capsid poliovirus proteins of the P2 region in extracts of infected cells were solubilized by SDS, separated by PAGE, electroeluted from the gels and used to immunize mice. The sera obtained were rendered monospecific by extensive absorption with proteins from uninfected cell extracts, and the spleen cells of these animals were used to establish antibody-secreting hybridomas. The monoclonal and the monospecific antibodies recognized enatured antigen (proteins 2C, 2BC and P2) in ELISA, immunoblot and immunoprecipitation tests and also combined with the native proteins of the P2 region in immunoprecipitation. In addition, the antibodies could be used successfully for immunofluorescence and electron microscopic immunocytochemistry