Extraction and Cleanup of XAD-2 Resin Cartridges for

This SOP is applicable to the extraction and analysis of polychlorinated biphenyl compounds extracted from fresh water using XAD-2 polymeric resin cartridges. The target compounds which can be determined by this method are, generally speaking, the polychlorinated biphenyls (PCBs) found in Aroclors 1232, 1248, and 1262 and trans-Nonachlor. It may be applied to other compounds once acceptable method recovery has been demonstrated. Up to 200 liters of filtered freshwater is passed through a glass column containing approximately 400 grams of pre-cleaned XAD-2 resin at a flow rate of approximately 1 liter per minute. This resin has been shown to efficiently extract non-polar organic compounds, including PCBs, from freshwater. The columns are sealed, labeled, and stored under refrigeration until analysis. At analysis the resin is transferred from the column to an extraction apparatus and rinsed with acetone to remove external water. The surrogate compounds are then added and the resin is then extracted with acetone to remove organics and interstitial water. Next the resin is extracted with a mixed solvent of 50 % hexane 50 % acetone to remove the remaining organic compounds. The acetone rinse and acetone extract are combined in a separatory funnel, 300 mL of reagent water is added, and the mixture is then extracted once with 200 mL of hexane then twice with 100 mL o