Add to ORKGBackground: Methods for analysis of the single-nucle-otide polymorphism (SNP) known as factor V Leiden (FVL) are described. The technique provides rapid, highly accurate detection of the point mutation that encodes for replacement of arginine-506 with glutamine. After formal assay qualification, 758 clinical samples that had previously been analyzed by the InvaderTM Monoplex Assay were tested as research samples in a commercial clinical laboratory. Methods: Primers specific for factor V (FV) were pre-pared, and PCR was performed. Samples were analyzed using the NanoChip ® Molecular Biology Workstation with fluorescently labeled reporters for wild-type and SNP sequences. Results: Of the 635 samples classified by the Thir
An optimized microchip electrophoresis system for mutation detection by tandem SSCP and heteroduplex...
mutation have been shown to have an increased risk of developing venous thromboembolism. Our aim was...
A nucleic acid photocross-linking technology was used in the development of a direct assay for facto...
BACKGROUND: Microelectronic DNA chip devices represent an emerging technology for genotyping. We dev...
sent an emerging technology for genotyping. We devel-oped methods for detection of single-nucleotide...
We have previously reported the use of a novel mini-sequencing protocol for detection of the factor ...
Activated protein C resistance (APCR), the most common risk factor for venous thrombosis, is the res...
cons with the DNA dye LCGreenTM I was recently introduced as a homogeneous, closed-tube method of ge...
One major goal of genetic research is to understand the role of genetic variation. By far the most c...
rz.uni-sb.de) Resistance of coagulation factor V to activated protein C is caused by a point mutatio...
With the identification of common single locus point mutations as risk factors for thrombophilia, ma...
WOS: 000458222000092PubMed ID: 30552928Single nucleotide polymorphisms (SNPs) are the most common ty...
V, produces an Arg506Gln substitution and is the most common genetic risk factor for venous thrombos...
Digital single-nucleotide polymorphism (SNP) analysis is developed to amplify a single tem-plate fro...
Copyright © 2014 James H. Smith et al.This is an open access article distributed under the Creative ...
An optimized microchip electrophoresis system for mutation detection by tandem SSCP and heteroduplex...
mutation have been shown to have an increased risk of developing venous thromboembolism. Our aim was...
A nucleic acid photocross-linking technology was used in the development of a direct assay for facto...
BACKGROUND: Microelectronic DNA chip devices represent an emerging technology for genotyping. We dev...
sent an emerging technology for genotyping. We devel-oped methods for detection of single-nucleotide...
We have previously reported the use of a novel mini-sequencing protocol for detection of the factor ...
Activated protein C resistance (APCR), the most common risk factor for venous thrombosis, is the res...
cons with the DNA dye LCGreenTM I was recently introduced as a homogeneous, closed-tube method of ge...
One major goal of genetic research is to understand the role of genetic variation. By far the most c...
rz.uni-sb.de) Resistance of coagulation factor V to activated protein C is caused by a point mutatio...
With the identification of common single locus point mutations as risk factors for thrombophilia, ma...
WOS: 000458222000092PubMed ID: 30552928Single nucleotide polymorphisms (SNPs) are the most common ty...
V, produces an Arg506Gln substitution and is the most common genetic risk factor for venous thrombos...
Digital single-nucleotide polymorphism (SNP) analysis is developed to amplify a single tem-plate fro...
Copyright © 2014 James H. Smith et al.This is an open access article distributed under the Creative ...
An optimized microchip electrophoresis system for mutation detection by tandem SSCP and heteroduplex...
mutation have been shown to have an increased risk of developing venous thromboembolism. Our aim was...
A nucleic acid photocross-linking technology was used in the development of a direct assay for facto...