Measurement of Plasma Estradiol- 17/3 by

We describe a simple, solid-phase chemiluminescence immunoassay for the measurement of estradiol-17f in extracts of peripheral venous plasma. The method has similar sensitivity, specificity, precision, and accuracy to a conventional radioimmunoassay with a tritiated antigen. An immunoglobulin G fraction of monoclonal antibodies to estradiol-6-carboxymethyl oxime-bovine serum albumin is passively adsorbed onto the walls of polypropylene tubes. The labeled antigen is estradiol-6-carboxymethyl oxime-aminobutylethyl isoluminol. After the binding re-action (1 hat 22 #{176}C),the solution is removed by aspiration and the antibody-bound fraction is washed once with buffer (400 tL). Sodium hydroxide (5 mol/L, 300 ILL) is added and the mixture incubated for 30 mm at 37 #{176}C.Luminescence is initiated by oxidation of the label with microperox