peritoneal disseminating foci of human colon cancer line Lovo

AIM: To study the effect of angiogenesis inhibitor TNP-470 on peritoneal dissemination of colon cancer in nude mice. METHODS: The MTT assay was used to evaluate the inhibitory effect of TNP-470 on human colon cancer cell line Lovo. Lovo cells were injected into the peritoneal cavity of BABL/C nu/nu mice and the models of peritoneal dissemination were developed. Thirty nude mice were randomly divided into control and TNP-470-treated group. In TNP-470-treated group, TNP-470 was injected subcutaneously every other day from day 1 until sacrifice or death (30 mg·kg-1). The control group received a sham injection of the same volume saline solution. RESULTS: In vitro, TNP-470 inhibited the growth of Lovo cells, with its IC50 at 2.14×102 µg·L-1. In vivo, TNP-47