Kinetic Measurement of Glucose with a Centrifugal Analyzer; Hexokinase and Glucose Oxidase Procedures Compared

We describe automated methods for a centrifugal analyzer for plasma glucose analysis by a fixed-time kinetic ap-proach. Results by the two methods correlated well, and their precision (day-to-day) is reflected by coefficients of variation of less than 2.5 % for each method. The application of a nonlinear fixed-time kinetic approach to the hexokinase (ATP:D-hexose-6-phosphotransferase, EC 2.7.1.1) method for plasma glucose has been mentioned by Tiffany et al. (1). We have adapted and evaluated linear fixed-time kinetic methods for plasma glucose with use of both the hexokinase/glucose-6-phosphate dehydrogenase (D-glu-cose-6-phosphate:NADP 1-oxidoreductase, EC 1.1.1.49) coupled enzyme assay (2) and the glucose oxidase (-D-glu-cose:oxygen oxidoreductase, EC 1.1.3.4) method of Trinder (3). Materials and Methods Apparatus. We used a centrifugal analyzer, the Aminc