Experimental Studies on Capturing Human Leukocytes with Cell Immuno-Chip, Yuzhi Yang,1 Chunxiu Zhang,1,2

Flow cytometry and monoclonal antibody technology have led to an active field of cellular analysis, particularly in differentiation of various human leukocyte cells and tumor cells by identification of their surface antigens. For example, the immunophenotypic analysis of acute leuke-mia by flow cytometry has become a powerful tool for proper identification of myeloid or lymphoid lineage and has been widely used in clinical laboratories (1, 2). Meanwhile, other technologies based on the specific af-finity between the immobilized antibody and the associ-ated cell surface antigens have been under development, such as the magnetic sphere immobilized with antibodies used in hematologic research and therapy (3, 4). How-ever, most of these technologies are limited in their potential for high-throughput analysis. Recently, the Lu