Using a combined strategy of random mutagenesis of haloalkane dehalogenase and genetic engineering of a chloropropanol-utilizing bacterium, we constructed an organism that is capable of growth on 1,2,3-trichloro-propane (TCP). This highly toxic and recalcitrant compound is a waste product generated from the manu-facture of the industrial chemical epichlorohydrin. Attempts to select and enrich bacterial cultures that can degrade TCP from environmental samples have repeatedly been unsuccessful, prohibiting the development of a biological process for groundwater treatment. The critical step in the aerobic degradation of TCP is the initial dehalogenation to 2,3-dichloro-1-propanol. We used random mutagenesis and screening on eosin-methylene blu...
Purpose 1,2,3-Trichloropropane (TCP) is a persistent groundwater pollutant and a suspected human car...
The capacity of an aerobic bacterial strain to degrade several chlorinated aliphatic hydrocarbons (C...
The bacterial degradation and detoxification of chlorinated xenobiotic compounds requires the produc...
Using a combined strategy of random mutagenesis of haloalkane dehalogenase and genetic engineering o...
Using a combined strategy of random mutagenesis of haloalkane dehalogenase and genetic engineering o...
1,2,3-Trichloropropane (TCP) is a toxic compound that is recalcitrant to biodegradation in the envir...
Trihalogenated propanes are toxic and recalcitrant organic compounds. Attempts to obtain pure bacter...
Trihalogenated propanes are toxic and recalcitrant organic compounds. Attempts to obtain pure bacter...
Trihalogenated propanes are toxic and recalcitrant organic compounds. Attempts to obtain pure bacter...
The gram-negative bacterium Pseudomonas cichorii 170, isolated from soil that was repeatedly treated...
The gram-negative bacterium Pseudomonas cichorii 170, isolated from soil that was repeatedly treated...
This dissertation describes the application of protein engineering and metabolic engineering approac...
We used directed evolution to obtain enantiocomplementary haloalkane dehalogenase variants that conv...
We used directed evolution to obtain enantiocomplementary haloalkane dehalogenase variants that conv...
1,2,3-Trichloropropane (TCP) is a persistent groundwater pollutant and a suspected human carcinogen....
Purpose 1,2,3-Trichloropropane (TCP) is a persistent groundwater pollutant and a suspected human car...
The capacity of an aerobic bacterial strain to degrade several chlorinated aliphatic hydrocarbons (C...
The bacterial degradation and detoxification of chlorinated xenobiotic compounds requires the produc...
Using a combined strategy of random mutagenesis of haloalkane dehalogenase and genetic engineering o...
Using a combined strategy of random mutagenesis of haloalkane dehalogenase and genetic engineering o...
1,2,3-Trichloropropane (TCP) is a toxic compound that is recalcitrant to biodegradation in the envir...
Trihalogenated propanes are toxic and recalcitrant organic compounds. Attempts to obtain pure bacter...
Trihalogenated propanes are toxic and recalcitrant organic compounds. Attempts to obtain pure bacter...
Trihalogenated propanes are toxic and recalcitrant organic compounds. Attempts to obtain pure bacter...
The gram-negative bacterium Pseudomonas cichorii 170, isolated from soil that was repeatedly treated...
The gram-negative bacterium Pseudomonas cichorii 170, isolated from soil that was repeatedly treated...
This dissertation describes the application of protein engineering and metabolic engineering approac...
We used directed evolution to obtain enantiocomplementary haloalkane dehalogenase variants that conv...
We used directed evolution to obtain enantiocomplementary haloalkane dehalogenase variants that conv...
1,2,3-Trichloropropane (TCP) is a persistent groundwater pollutant and a suspected human carcinogen....
Purpose 1,2,3-Trichloropropane (TCP) is a persistent groundwater pollutant and a suspected human car...
The capacity of an aerobic bacterial strain to degrade several chlorinated aliphatic hydrocarbons (C...
The bacterial degradation and detoxification of chlorinated xenobiotic compounds requires the produc...