The Use of Fractal Analysis for the Det

The advances in digital image recording techniqu made these technologies suitable and available for vario igital techniques are now applicable in those segments of ses of image evaluation prevailed until nowadays. There exist many ways of biological specimen image analysis and they are readily available as complete commercial software products. Anyway le to biological and microbiological research. Software a t our faculty. Originally, it has been designed for the imag ables the user to make various correction of the captured im both harmonic and fractal analysis. At the same time, it gical specimens as well. The harmonic and fractal analysis of the imag basic methods for the image analysis. This analysis can be hich is commonly available [1], [2], [3]. However, this, and therefore it is not applicable generally. Just this m gical specimens for the determination of diameter distribu It has been shown that under certain circumstanc of a microscopic specimen. The circumstances are: • cells have to be spherical or ellipsoidal, • cells have to be similar in size, • cells have to be coloured different from the ba Under these conditions it is possible to determin the size distribution. The image was taken by recording equipment, which consists of optical microscope SM-6, digital camera SONY and PC. The microscope magnification, resolution of digital camera gives the connection between image size and studied sample size (100 µm per 514 pixels). According to the experience from previous work we tried to widen the field of application of this method. During the previous work, it was possible to determine the number and size of cells fairly correctly. The samples contained suitable estimated number of cells (100) of appropriate size (35 pixels). When compared with the standard manual method of counting by Bürker box, the error was less than 10 %. During the analysis of captured image, it is necessary to respect this optimal number and the deviation from correct values determined manually. Our proposed analytical process consists of the following steps