AIM: To investigate the transactivating effect of hepatitis C virus (HCV) core protein and to screen genes transactivated by HCV core protein. METHODS: pcDNA3.1(-)-core containing full-length HCV core gene was constructed by insertion of HCV core gene into EcoRI/BamHI site. HepG2 cells were cotransfected with pcDNA3.1(-)-core and pSV-lacZ. After 48 h, cells were collected and detected for the expression of b-gal by an enzyme-linked immunosorbent assay (ELISA) kit. HepG2 cells were transiently transfected with pcDNA3.1(-)-core using Lipofectamine reagent. Cells were collected and total mRNA was isolated. A subtracted cDNA library was generated and constructed into a pGEM-Teasy vector. The library was amplified with E. coli strain JM109. The ...
BACKGROUND: The liver disease expression in chronic hepatitis C patients is variable and may partial...
AbstractHepatitis C virus (HCV) Core protein is implicated in the development of hepatocellular carc...
Hepatitis C virus (HCV) is an RNA virus that causes chronic infection, which can lead to hepatocellu...
Background: Worldwide, hepatitis C virus (HCV) infection is a serious public health disease unlike h...
<p>(A) Schematic representation of constructs used for the eukaryotic expression of core from the p3...
Hepatitis C virus (HCV) is a major worldwide cause of acute and chronic hepatitis, cirrhosis, and he...
Background and Objectives: Hepatitis C virus (HCV) chronically infects around 200 million people wo...
A tetracycline-regulated system was used to generate cell lines allowing tightly controlled expressi...
AbstractA tetracycline-regulated system was used to generate cell lines allowing tightly controlled ...
Hepatitis C viruses (HCV) affect 170 million patients, but only a minority of patients develop sympt...
Background: Hepatitis C virus (HCV) infection is one of the most prevalent infectious diseases resp...
The nucleocapsid core protein of hepatitis C virus (HCV) has been shown to trans-act on several vira...
Abstract: Hepatitis C virus (HCV) is a member of the family Flaviviridae, and the prin-cipal cause o...
Background & Objectives: The hepatitis C virus possesses an alternative reading frame overlapping t...
AbstractAlthough great progress has been made in the characterization of the biochemical and biologi...
BACKGROUND: The liver disease expression in chronic hepatitis C patients is variable and may partial...
AbstractHepatitis C virus (HCV) Core protein is implicated in the development of hepatocellular carc...
Hepatitis C virus (HCV) is an RNA virus that causes chronic infection, which can lead to hepatocellu...
Background: Worldwide, hepatitis C virus (HCV) infection is a serious public health disease unlike h...
<p>(A) Schematic representation of constructs used for the eukaryotic expression of core from the p3...
Hepatitis C virus (HCV) is a major worldwide cause of acute and chronic hepatitis, cirrhosis, and he...
Background and Objectives: Hepatitis C virus (HCV) chronically infects around 200 million people wo...
A tetracycline-regulated system was used to generate cell lines allowing tightly controlled expressi...
AbstractA tetracycline-regulated system was used to generate cell lines allowing tightly controlled ...
Hepatitis C viruses (HCV) affect 170 million patients, but only a minority of patients develop sympt...
Background: Hepatitis C virus (HCV) infection is one of the most prevalent infectious diseases resp...
The nucleocapsid core protein of hepatitis C virus (HCV) has been shown to trans-act on several vira...
Abstract: Hepatitis C virus (HCV) is a member of the family Flaviviridae, and the prin-cipal cause o...
Background & Objectives: The hepatitis C virus possesses an alternative reading frame overlapping t...
AbstractAlthough great progress has been made in the characterization of the biochemical and biologi...
BACKGROUND: The liver disease expression in chronic hepatitis C patients is variable and may partial...
AbstractHepatitis C virus (HCV) Core protein is implicated in the development of hepatocellular carc...
Hepatitis C virus (HCV) is an RNA virus that causes chronic infection, which can lead to hepatocellu...
Add to ORKG