Journal of Leukocyte Biology 40:589-600 (1986) The Immunoregulatory Nature of Iron. II.

Previously, we presented preliminary evidence that supported our hypothesis for the immunoregulatory nature of iron [7. The objective of the present work was to test that hypothesis in greater detail. Our approach was to examine the effect that iron had on the expression of the surface markers on lymphocytes that had been activated by pokeweed mitogen (PWM). The two categories of lymphoid surface molecules were enumerated on those cells; first were those that identify T lymphocytes and second, those that appear on the membrane of T cells following activation. The results, as regards T cell-associated mole-cules, demonstrated that iron suppresses the expression of the molecules identified by the monoclonal antibodies OKT3 and OKT4. It suppressed expres-sion of the T4 molecule in PWM-activated cells (30.6 % ± 4.5; n = 5) com-pared with untreated but activated cells (52.2 % ± 2.9; n = 5; P = 1.9 x iO-) resulting in a reduced helper:suppressor T cell ratio from 2.2 ± 0.4 to 1.2 ± 0.3. With regard to activation-associated lymphocyte markers, iron significantly enhanced expression of the receptor for transferrin as identified by the monoclonal antibody, OKT9. However, it failed to change significantly the expression of three other activation-associated markers, namely, Ia, T10, and the receptor that forms thermostable erythrocyte-rosettes (TE-R) with sheep red blood cells (SAC). We conclude from those results that iron has a differential immunoregulatory influence on the expression of certain lympho-cyte surface molecules on actively dividing lymphocytes. Key words: transferrin receptor, immune regulation, T lymphocyte