In a URA3 /5-FOA-based dosage-suppressor screen, we isolated a plasmid containing the little-characterized ORF YJL055W. Further analysis showed that this gene did not suppress the mutation of interest. Instead, overexpression of Yjl055Wp directly suppressed the non-viability of URA3+ cells in the presence of 5-FOA. Overexpression of Yjl055Wp also suppressed the lethality induced by 5-FU, but deletion of YJL055W had no detectable effect on resistance to either 5-FOA or 5-FU. Based on these observations and a previous report that a yjl055wmutant has increased sensitivity to purine-analogue mutagens, we suggest that Yjl055Wp may function in one of several pathways for the detoxification of base analogues. However, its precise mechanism of acti...
<p>(A) Wild type cells. 5-FU is taken up and converted to 5-FUMP, which competes with freshly synthe...
Resistance to 5-fluorocytosine (5-FC) has been poorly investigated in the yeast Candida glabrata. Th...
Purine and pyrimidine bases are the central precursors of DNA and RNA and theirintracellular concent...
5-Fluorouracil (5-FU) is an anticancer drug and pyrimidine analogue. A problem in 5-FU therapy is ac...
<div><p>5-Fluorouracil (5-FU) is an anticancer drug and pyrimidine analogue. A problem in 5-FU thera...
5-Fluorouracil (5-FU) is an anticancer drug and pyrimidine analogue. A problem in 5-FU therapy is ac...
5-Fluorouracil (5-FU) is an anticancer drug and pyrimidine analogue. A problem in 5-FU therapy is ac...
Purine and pyrimidine analogues have important uses in chemotherapies against cancer, and a better u...
<p>(A) Dependency of the 5-FU resistance conferred by different plasmids on <i>URA2</i> function. A ...
<p>Each plasmid was transformed into yeast knockout strains where one of the other resistance genes ...
<p>All mutants tested were <i>kanMX</i> gene disruptions in the BY4742 genetic background. Since BY4...
The purpose of this work was to identify the function of an open reading frame called YBL042, found ...
The purpose of this work was to identify the function of an open reading frame called YBL042, found ...
5-fluorouracil (5-FU) is a widely used anticancer drug thatdisruptspyrimidinenucleotidepool balances...
The putative translation initiation factor 5A (eIF5A) is a small protein, highly conserved and essen...
<p>(A) Wild type cells. 5-FU is taken up and converted to 5-FUMP, which competes with freshly synthe...
Resistance to 5-fluorocytosine (5-FC) has been poorly investigated in the yeast Candida glabrata. Th...
Purine and pyrimidine bases are the central precursors of DNA and RNA and theirintracellular concent...
5-Fluorouracil (5-FU) is an anticancer drug and pyrimidine analogue. A problem in 5-FU therapy is ac...
<div><p>5-Fluorouracil (5-FU) is an anticancer drug and pyrimidine analogue. A problem in 5-FU thera...
5-Fluorouracil (5-FU) is an anticancer drug and pyrimidine analogue. A problem in 5-FU therapy is ac...
5-Fluorouracil (5-FU) is an anticancer drug and pyrimidine analogue. A problem in 5-FU therapy is ac...
Purine and pyrimidine analogues have important uses in chemotherapies against cancer, and a better u...
<p>(A) Dependency of the 5-FU resistance conferred by different plasmids on <i>URA2</i> function. A ...
<p>Each plasmid was transformed into yeast knockout strains where one of the other resistance genes ...
<p>All mutants tested were <i>kanMX</i> gene disruptions in the BY4742 genetic background. Since BY4...
The purpose of this work was to identify the function of an open reading frame called YBL042, found ...
The purpose of this work was to identify the function of an open reading frame called YBL042, found ...
5-fluorouracil (5-FU) is a widely used anticancer drug thatdisruptspyrimidinenucleotidepool balances...
The putative translation initiation factor 5A (eIF5A) is a small protein, highly conserved and essen...
<p>(A) Wild type cells. 5-FU is taken up and converted to 5-FUMP, which competes with freshly synthe...
Resistance to 5-fluorocytosine (5-FC) has been poorly investigated in the yeast Candida glabrata. Th...
Purine and pyrimidine bases are the central precursors of DNA and RNA and theirintracellular concent...