Plasma F2-Isoprostanes and Coronary Artery Calcification: The CARDIA Study

cells may initiate and enhance the early development of cardiovascular disease. Method and Results: We assayed F2-isoprostanes, oxi-dation products of arachidonic acid, by gas chromatog-raphy–mass spectrometry in a biracial cohort of 2850 young healthy adult men and women. Coronary artery calcification (CAC), a component of coronary artery atherosclerosis, was detectable in 10 % of the cohort and appeared to be in its initial stages (Agatston scores <20 in 47 % and <100 in 83 % of CAC-positive participants). After adjusting for sex, clinical site, age, and race, the presence of any CAC was 24 % more likely among those with high vs low concentrations of F2-isoprostanes [odds ratio (OR) 1.24 per 92.2 pmol/L (32.7 ng/L; 1 SD of F2-isoprostanes); 95 % confidence interval (CI), 1.09