SHORT REPORT: IDENTIFICATION IN TRIATOMINE VECTORS OF FEEDING SOURCES AND TRYPANOSOMA CRUZI VARIANTS BY HETERODUPLEX ASSAY AND A MULTIPLEX MINIEXON POLYMERASE CHAIN

Abstract. Feeding sources of triatomine vectors (Triatoma longipennis) collected in peridomiciles in Mexico were identified by a heteroduplex assay developed with triatomine blood meals. Trypanosoma cruzi parasites were also characterized in the same blood meal samples by multiplex-polymerase chain reaction assay of mini-exon gene inter-genic regions. The main blood meal source was from rats, but the bugs were able to feed on a wide variety of hosts, and human blood meals were identified. Trypanosoma cruzi was the only flagellate species identified in the blood meals. All populations belong to the T. cruzi I lineage, a result that is consistent with the previously assumed predominance of this lineage in Mexico. This combination of blood meal and T. cruzi lineage identification provides a powerful tool for understanding T. cruzi transmission cycles. The presence and colonization of human habitats by tri-atomines, the obligatory hematophagous vectors of Chagas disease, rely upon accessibility to blood meal sources. There-fore, identification of feeding sources is very helpful in char-acterizing feeding habits of the different vectors populations (wild, peridomestic, and domestic), identifying the mamma-lian species that may favor colonization and long-term infes