Inhalation tolerance study of p-aramid respirable fibershaped particulates (RFP) in rats. Toxicol

This study was designed to assess the lung clearance function in rats after subchronic exposure to p-aramid respirable fiber-shaped particulates (RFP). Male Wistar rats were exposed 6 hrs/day, 5 days/week for 3 months to 50, 200, and 800 RFP/ml measured by scanning electron microscopy (SEM). Recovery effects were fol-lowed up through 9 months postexposure. The retention of RFP (length> 5 mm) was about 25 3 106 RFPs per lung in the low dose group after 3 months of exposure. The corresponding values in the medium and high dose groups amounted to overproportionally higher values of 122 3 106 and 576 3 106 RFPs per lung, respec-tively. A decrease in the length of the retained RFPs over the 9-month recovery period was observed, indicating a breakage of long fibrils. Alveolar clearance half-times measured by g tracers indicated a dust overloading of lungs for the high dose group at 0 and 3 months postexposure. Bronchoalveolar lavage parameters revealed that p-aramid RFPs induced pronounced inflammatory effects in the high and medium dose groups. Histopathologically, slight fibrotic and hyperplastic lesions were observed in the me-dium and high dose groups directly after the end of exposure. The findings at the 3-month postexposure interval resulted in a reduc-tion of inflammatory changes in the medium and high dose groups compared to the sacrifices upon cessation of exposure. No his-topathologic effects were detected in the low dose group. In the high dose group the maximum functionally tolerated dose was exceeded. The No Observed Adverse Effect Level (NOAEL) of RFP was 50 RFP/ml as measured by SEM