During a Subcutaneous Nonspecific Inflammation in the Mouse

The oxidative metabolism of remote phagocytes has been studied during the course of an acute nonspecific inflammation in the mouse. The bone marrow cells (BMC) from mice bearing a polyacrylamide-microbead-induced granuioma (Biogels P4 and P100) show a transient striking decrease of their chemiluminescence (CL) response to opson-ized zymosan. This decreased oxidative response occurs between the third and 72nd hr, with a minimum observed at the 24th hr. Bone marrow granulocytes are assessed to represent the main chemiluminescent BMC compartment, using complement-mediated cytotoxicity assays with a monoclonai antimouse granulocyte antibody. Biogel P4-treated mice, but not Biogel P1,,-treated ones, show at the 24th hr a significantly decreased percentage of segmented bone marrow granulocytes (- 45%, p <.001). The accurate mechanisms involved in this transient suppression of stimulus-induced CL produced by BMC remain to be elucidated. Key words: granuiocytes, polyacrylamide-bead-induced granulom