localization of Sex-lethal protein

The Drosophila ovarian tumour gene is required at multiple times in the germline for oogenesis. A second gene, Sex-lethal, controls sex determination in the soma and also has a separate germline function affect-ing similar oogenic stages as ovarian tumour. We demonstrate that ovarian tumour is not required for early Sex-lethal gene expression in the female germline, as had been previously reported. Instead, we provide evidence that ovarian tumour has a specific role in the developmentally regulated accumulation of SEX-LETHAL protein within the cytoplasm and nucleus. Furthermore, the examination of nurse cell polytene chromosomes produced by certain ovarian tumour mutations showed that SEX-LETHAL protein can asso-ciate with discrete chromosomal sites in the germline and that this pattern appears to change as the egg chamber matures. This is the first indication that SEX-LETHAL is capable of direct physical interactions with chromosomes (albeit in a mutant background) and is consistent with the developmentally regulated nuclear localization of SEX-LETHAL being important for oogenesis