Isolation and characterization of canine adiposederived mesenchymal stem cells

This study is the first documentation of the isolation and extensive characterization ofmesenchymal stemcells fromcanine adipose tissue.Methodspreviously usedby our group to isolate anddifferentiate humanadipose– derived mesenchymal stem cells (hAD-MSCs) have been modified and optimized for derivation of similar cells from canine adipose tissues. The canine adipose tissue–derived mesenchymal stem cells (cAD-MSCs) showed lower proliferation ability and were refractory to osteogenic and adipogenic differentiation under conditions employed to differentiate hAD-MSCs. The differentiation of cAD-MSCs into osteoblasts and adipocytes was effectively achieved under modified conditions, by using laminin-coated plates and peroxi-some proliferative activated receptor, gamma (PPARg) ligands, respectively. The formation of micromass was sufficient to induce chondrogenesis, unlike hAD-MSCs, which require transforming growth factor beta (TGF-b). These cells displayed anchorage-independent growth in soft agar, and their colony-forming effi-ciency in plastic was comparable with human counterparts. The cAD-MSCs expressed genes associated with pluripotency, while their differentiated progeny expressed appropriate lineage-specific genes. The optimi-zation of growth and differentiation of cAD-MSCs should facilitate future stem cell–based reparative and regenerative studies in dogs. The dog is a promising biomedical model that is suitable for evaluation of novel therapies such as those employing stem cells in experimental and in spontaneous disease settings