Antioxidative Property and LC/MS Analysis of Apis Honey Samples Collected from Different Geographical Locations of India

Abstract: The antioxidative property analysis was carried out for six different honey samples collected from different apiaries in India. The antioxidative property was determined on the basis of total phenol content in the six honey samples and their antiradical scavenging activity (DPPH assay). From the honey samples analysed, honey sample AP3 was found to have maximum antioxidative property. The total phenol content of honey sample AP3 was found to be 1.1 mg/L and the antiradical scavenging activity was found to be about 63.63%. Hence further honey sample AP3 was subjected to LC/MS analysis in order to know its composition. From the LC/MS analysis it was found that honey sample AP3 contains L- ethionine, N- acetyl glycine, oxamic acid, succinimide, xanthurenic acid, brivudin, histidine, quisqualic acid, quanosine and sissotrine. As the honey sample AP3 has significant antioxidative property, it can act as a good antimicrobial agent, effective wound healer which has no side effects, food preservative which is rich in nutrition. Key words: Antioxidative property Apis honey Total phenol content Antiradical scavenging LC/MS INTRODUCTION The objective of this study was therefore to identify Honey is a natural sweet substance produced from from different apiaries based on its total phenol content the nectar of blossoms. It has high nutritional value. and antiradical scavenging activity by DPPH method. The Gluconic acid is the predominant acid present in honey. honey sample that showed good antioxidative propert