ESTIMATION OF GENETIC DIVERSITY IN ALBUS LUPIN (L UPINUS ALBUS L.) USING DART AND GENIC MARKERS

Ninety four accessions of white lupin (Lupinus albus L.) representing local and foreign cultivars, landraces and advanced breeding lines were analysed for allelic diversity with Intron-Targeted Amplified Polymorphisms (ITAPs), SSR motifs, and DArT markers developed from Lupinus species. A total of 295 and 50 alleles were identified using DArT and ITAP markers respectively. The number of ITAP/SSR alleles per locus ranged from one to eight. A principal coordinate analysis indicated a high level of genetic diversity in the germplasm and showed that both the genic and the DArT markers used in this study are suitable for estimation of genetic diversity. Landrace accessions from Ethiopia formed a distinct and separate grouping. Australian cultivars and breeding lines were clustered and tended to be distinct from European landraces. These findings will allow us to select appropriate parents, and broaden the genetic base of the white-lupin breeding program