REDUCED GLUCOSE DEGRADATION PRODUCTS IN BICARBONATE/LACTATE-BUFFERED PERITONEAL DIAL YSIS SOLUTIONS PRODUCED IN TWO-CHAMBERED BAGS

.Objectives: The aims of the current study were: (1) to determine the effects of peritoneal dialysis (PD) solutions at different glucose concentrations on the growth of cultured cells; (2) to determine whether a bicarbonate / lactate-based solution, as a result of the configuration of its components during heat sterilization in a two-chambered bag, was lower in glucose degradation products than a corresponding lactate-based PD solution; and (3) to determine whether lower glucose degradation corresponded to a decreased inhibition of cell growth..Design: Growth inhibition of cells exposed to lactatebased PD solutions at three different glucose concentrations was determined. Bicarbonate/lactate-based and lactate-based solutions at high glucose concentration (3.86%) were further analyzed for presence of glucose degradation products and inhibition of cell growth..Methods: Cell growth was determined by neutral red uptake, measured by optical density at 540 nm. Glucose degradation t