Switch mating type Mate

hybrid diploid a α a α a/α a/α Assay fitness and sporulation Supplemental Figure S1 Figure S1. Experimental protocol for constructing homozygous F2 hybrid diploids S. bayanus α cells were crossed to S. cerevisiae a cells to generate F1 hybrid cells. The hybrid diploids were then induced to sporulate and 40 viable F1 spores were selected and examined by FACS to confirm that they were haploid. The selected haploids were switched to the opposite mating types and mated with the original clones to generate homozygous F2 hybrid diploids. The homozygous diploids were assayed for their fitness and sporulation frequency. Ch r