Molecular cloning and characterization of rat Pomt1 and Pomt2

Mammalian O-mannosylation, although an uncommon type of protein modification, is essential for normal brain and mus-cle development. Defective O-mannosylation causes congeni-tal muscular dystrophy with abnormal neuronal migration [Walker–Warburg syndrome (WWS)]. Here, we have identi-fied and cloned rat Pomt1 and Pomt2, which are homologues of human POMT1 and POMT2, with identities of 86 and 90%, respectively, at the amino acid level. Coexpression of both genes was found to be necessary for enzymatic activity, as is the case with human POMT1 and POMT2. Northern blot and reverse transcriptase polymerase chain reaction (RT–PCR) analyses revealed that rat Pomt1 and Pomt2 are expressed in all tissues but most strongly in testis. In situ hybridization histochemistry of rat brain revealed that Pomt1 and Pomt2 mRNA are coexpressed in neurons (dentate gyrus and CA1-CA3 region of the hippocampus and cerebellar Purkinje cells). Two transcription-initiation sites were observed in rat Pomt2, resulting in two forms: a testis form and a somatic form. The two forms had equal protein O-mannosyl-transferase activity when coexpressed with rat Pomt1. Coex-pression studies also showed that the human and rat protein O-mannosyltransferases are interchangeable, providing further evidence for the closeness of their structures