Antigen Microarrays for Serodiagnosis of Infectious Diseases

has allowed the development of high-density nucleic acid and protein arrays that have increased the through-put of a variety of assays. We generated protein microar-rays by printing microbial antigens to simultaneously determine in human sera antibodies directed against Toxoplasma gondii, rubella virus, cytomegalovirus (CMV), and herpes simplex virus (HSV) types 1 and 2 (ToRCH antigens). Methods: The antigens were printed on activated glass slides with high-speed robotics. The slides were incu-bated first with serum samples and subsequently with fluorescently labeled secondary antibodies. Human IgG and IgM bound to the printed antigens were detected by confocal scanning microscopy and quantified with in-ternal calibration curves. Both microarrays and commer