Neutrophil maturation and activation determine anatomic site of clearance from circulation

Worthen. Neutrophil maturation and activation determine anatomic site of clearance from circulation. Am J Physiol Lung Cell Mol Physiol 281: L913–L921, 2001.—The long-term disposition of circulating neutrophils and the site of disappearance from circulation remain unclear. We investi-gated neutrophil localization in mice using 111In-labeled mu-rine peripheral blood neutrophils, mature bone marrow neu-trophils, and peritoneal exudate neutrophils to track in vivo localization of these different cell populations. Infused pe-ripheral neutrophils were found to localize equally between liver and marrow sites by 4 h (31.2 6 1.9 vs. 31.9 6 1.8%), whereas exudate neutrophils predominantly localized to liver (42.0 6 1.1%) and marrow-derived neutrophils to the marrow (65.9 6 6.6%) where they were found to localize predomi-nantly in the hematopoietic cords. Stimulation of marro