Analysis of Clinically Relevant Single-Nucleotide Polymorphisms by Use of Microelectronic Array Technology

sent an emerging technology for genotyping. We devel-oped methods for detection of single-nucleotide poly-morphisms (SNPs) in clinically relevant genes. Methods: Primer pairs, with one containing a 5-biotin group, were used to PCR-amplify the region encompass-ing the SNP to be interrogated. After denaturation, the biotinylated strand was electronically targeted to dis-crete sites on streptavidin-coated gel pads surfaces by use of a Nanogen Molecular Workstation. Allele-spe-cific dye-labeled oligonucleotide reporters were used for detection of wild-type and variant sequences. Meth-ods were developed for SNPs in genes, including factor VII, -globin, and the RET protooncogene. We geno-typed 331 samples for five DNA variations in the factor VII gene,>600 samples from patients with -thalasse