Phospholemman Transmembrane Structure Phospholemman transmembrane structure reveals potential interactions with Na+/K+-ATPase*

Phospholemman (PLM) is a 72-residue bitopic cardiac transmembrane protein, which acts as a modulator of the Na+/K+-ATPase, the Na+/Ca2+ exchanger, and possibly forms taurine channels in non-heart tissue. This work presents a high-resolution structural model obtained from a combination of site-specific infrared spectroscopy and experimentally constrained high-throughput molecular dynamics (MD) simulations. Altogether 37 experimental constraints including 9 long-range orientational constraints have been used during MD simulations in an explicit lipid bilayer/water system. The resulting tetrameric α-helical bundle has an average helix tilt of 7.3