Cardiac Effects of Carbon Monoxide and Ambient Particles in a Rat

Ambient air pollution is a complex mixture of particulate matter (PM) and gaseous pollutants such as carbon monoxide (CO). The effect of exposure to CO, alone or in combination with ambient PM, on arrhythmia incidence is unclear. To evaluate these effects, left-ventricular myocardial infarction was induced in Sprague-Dawley rats by thermocoagulation. Diazepam-sedated rats were exposed (1h) to eitherfilteredair (n540),CO(35ppm,n519), concentrated air particles (CAPs, median concentration5 350.5 mg/m3, n5 53), or CAPs and CO (CAPs median concentration5 318.2 mg/m3, n5 23), 12–18hafter surgery.Eachexposurewas immediately preceded and followed by a 1 h exposure to filtered air (pre-exposure andpost-exposure periods, respectively). The CO target dose of 35 ppm is related to the 1 h U.S. National Ambient Air Quality Standard. Surface electrocardiograms were recorded and heart rate and arrhythmia incidence were quantified. CO exposure reduced ventricular premature beat (VPB) frequency by 60.4 % ( p 5 0.012) during the exposure period compared to controls. This effect was modified by both infarct type and the number of pre-exposureVPBs, and was not mediated through changes in heart rate. Overall, CAPs exposure increased VPB frequency during the exposure period, but this effect did not reach statistical significance. This effect was modified by the number of pre-exposure VPBs. Overall, neither CAPs nor CO had any effect on heart rate, but CAPs increased heart rate in specific subgroups. No significant interactions were observed between the effects of CO and CAPs. In this animal model, the responses to CO and CAPs are distinctly different