ORIGINAL ARTICLE Cisplatin Causes Oxidation in Rat Liver Tissues: Possible Protective Effects of Antioxidant

Aims: In this study, we aimed to investigate the possible molecular mechanism of cisplatin hepatotoxicity and to establish whether some natural antioxidant foods, namely dried black grape and tomato, may provide protection against cisplatin hepatotoxicity. Materials and Methods: Twenty-eight rats were used throughout the study. Cisplatin was administered intraperitoneally (i.p.) in a single dose (10 mg/kg). Antioxidant food supplementation was started three days before cisplatin treatment. There were 7 animals in each group (control, cisplatin, cisplatin plus dried black grape and cisplatin plus tomato juice). Rats were sacrificed 72 h after the treatment. The livers were removed and prepared for the biochemical and histopathological investigations. Oxidant and antioxidant parameters were measured in liver tissues of the groups. Results: Malondialdehyde (MDA) level and xanthine oxidase (XO) activities were higher in the cisplatin group compared with the control values. Catalase (CAT) and glutathione peroxidase (GSH-Px) activities were higher but MDA level lower in the grape-supplemented group compared with the cisplatin group. In the histopathological examination, sinusoidal congestion, hydropic and vacuolar degeneration, extensive disorganization in hepatocytes, and significant fibrosis around central venules and expanded periportal areas were observed in the liver tissues from cisplatin-treated animals. In rats treated with cisplatin and fed wit