Comprehensive Analysis of Intracellular Metabolites by Capillary Electrophoresis Mass Spectrometry

In the post genome era, elucidation of gene function is a main focus. Multi-parallel analysis of mRNA and proteins are central to today’s functional genomics initiatives. However, these results do not provide direct interpretation of biological meanings on cellular and tissue events. Quantitative comprehensive analysis of metabolites compared with data obtained from mRNA and proteins analysis is crucial to elucidate a change in biological function. However, the comprehensive analysis of intracellular metabolites is still in the early stage, therefore only few studies have been reported 1-3. We describes here are novel methods for the quantitative comprehensive analysis of metabolites using capillary electrophoresis coupled to mass spectrometry (CE-MS). More than 30 positively charged metabolites including amino acids an