A retail and consumer phase model for exposure assessment of Bacillus cereus

An exposure assessment is conducted for psychrotrophic and mesophilic Bacillus cereus in a cooked chilled vegetable product. A model is constructed that covers the retail and consumer phase of the food pathway, using the output of a similar model on the industrial process as input. Microbial growth is the predominant process in the model. Variability in time and temperature during transport and storage is included in the model and different domestic refrigerator temperature distributions are compared. As an end point, probable levels of B. cereus colony forming units (cfu) in packages of vegetable purée are predicted at the moment the consumer takes the product from its refrigerator, that is prior to a cooking process. The psychrotrophic strain is predicted to end up above a threshold level of 105 cfu/g in 0.9 % to 6.3 % of the vegetable purée packages, depending on domestic refrigerator temperature. Accounting for spoilage this reduces to 0.3 % to 2.4%. Even if the purée is stored at 4 jC in the domestic refrigerator and use-by-date (UBD) is respected, the threshold level may be passed. For the mesophilic strain the threshold level is rarely passed, but in contrast to the total viable count, the spore load at the end point is predicted to be higher than in the psychrotrophic strain. Our study illustrates how an exposure assessment model, which may be used in quantitative risk assessment, can integrate expertise in modelling, food processing and microbiology over the food pathway, and thus evaluate food safety, identify gaps in knowledge and compare risk management measures. As important gap