comprime. Analusis 1988;16:173-8. Improving Accuracy of Glucose Oxidase Procedure for Glucose Determinations on Discrete Analyzers

We describe a rapid kinetic glucose oxidase (EC 1.1.3.4) procedure for quantifying glucose. Glucose oxidase con-centration was reduced from the more usual 20 kU/L to 4 kU/L, and pH was reduced from 7.0 to 6.6. Potassium ferrocyanide (20 moVL) and ascorbate oxidase (1 kU/L) were incorporated in the procedure.The assay results vary linearly with glucose concentration from 0 to 50 mmol/L and are unaffected by bilirubin concentrations 600 imol/L, hemoglobin c12 g/L, lntralipid#{174}4 g/L, urate 1 mmol/L, and ascorbate 2.0 mmol/L. The assay is readily adaptable to most open-system analyzers. Additional K.yphras.s: peroxidase. kinetic enzyme analysis Methods for measuring glucose by using glucose oxi-dase (EC 1.1.3.4) and peroxidase (EC 1.11.1.7) have been in existence for more than 25 years. The initia