Human and mouse oligonucleotide-based array CGH

Array-based comparative genomic hybridization is a high resolution method for measuring chromo-somal copy number changes. Here we present a validated protocol using in-house spotted oligonuc-leotide libraries for array comparative genomic hybridization (CGH). This oligo array CGH platform yields reproducible resultsand iscapable ofdetecting single copy gains, multi-copy amplifications as well as homozygous and heterozygous deletions as small as 100 kb with high resolution. A human oligonuc-leotide library was printed on amine binding slides. Arrays were hybridized using a hybstation and analysed using BlueFuse feature extraction software, with.95 % of spots passing quality control. The protocol allows as little as 300 ng of input DNA and a 90 % reduction of Cot-1 DNA without compromising quality. High quality results have also been obtained with DNA from archival tissue. Finally, in addition to human oligo arrays, we have applied the protocol successfully to mouse oligo arrays. We believe that this oligo-based platform using ‘off-the-shelf ’ oligo libraries provides an easy accessible alternative to BAC arrays for CGH, which is cost-effective, avail-able at high resolution and easily implemented for any sequenced organism without compromising the quality of the results